strain s aureus newman d2c strain  (ATCC)

Journal: Microbiology Spectrum

Article Title: A mechanistic understanding of the effect of Staphylococcus aureus VraS histidine kinase single-point mutation on antibiotic resistance

doi: 10.1128/spectrum.00095-25

Figure Lengend Snippet: Relationship between the drug concentrations and the bacterial burden. Dose-response curves of the tested antibiotics in the Newman WT and T331I mutant strain. Results obtained are in , and the parameters for the model are in Table S1.

Article Snippet: The drug-susceptible strain S. aureus Newman D2C strain (ATCC #25904) and its isogenic mutant VraS T331I were maintained in Luria-Bertani (LB) media at 37°C unless otherwise mentioned.

Techniques: Mutagenesis

Journal: Microbiology Spectrum

Article Title: NH125 Sensitizes Staphylococcus aureus to Cell Wall-Targeting Antibiotics through the Inhibition of the VraS Sensor Histidine Kinase

doi: 10.1128/spectrum.04861-22

Figure Lengend Snippet: The effect of the addition of 50 μM NH125 to the S. aureus Newman strain in the presence and absence of carbenicillin (Carb) and vancomycin (Van) on (A) bacterial growth kinetics, relative to the positive control (no antibiotics, no NH125), and (B) the fold change in the expression levels of vraSR , pbpB , and blaZ , relative to the positive-control culture (C). The data represent the mean ± SD ( n = 3), and statistical significance was calculated via a paired Student’s t test ( P < 0.05).

Article Snippet: Cultures of the S. aureus Newman D2C strain (ATCC number 25904) were diluted from overnight cultures to an OD 600 of approximately 0.1 and grown for 24 h with or without NH125, carbenicillin, or vancomycin, as indicated in Results.

Techniques: Positive Control, Expressing

Journal: Toxins

Article Title: Erianin against Staphylococcus aureus Infection via Inhibiting Sortase A

doi: 10.3390/toxins10100385

Figure Lengend Snippet: Inhibitory effects of erianin (different levels) against srtA from Staphylococcus aureus Newman D2C in vitro.

Article Snippet: S. aureus strain Newman D2C (ATCC25904) was commercially obtained from the American Type Culture Collection (ATCC) and used in this study.

Techniques: In Vitro

Journal: Toxins

Article Title: Erianin against Staphylococcus aureus Infection via Inhibiting Sortase A

doi: 10.3390/toxins10100385

Figure Lengend Snippet: Growth curves of Staphylococcus aureus strain Newman D2C with or without erianin and gene knockout mutant ΔsrtA in brain-heart infusion (BHI). Symbol ●, ■, ▲, ▼, ◆, ○ and □ represent gene knockout mutant ΔsrtA, Staphylococcus aureus strain Newman D2C culture with 0, 4, 16, 32, 64 and 128 μg/mL erianin, respectively.

Article Snippet: S. aureus strain Newman D2C (ATCC25904) was commercially obtained from the American Type Culture Collection (ATCC) and used in this study.

Techniques: Gene Knockout, Mutagenesis

Journal:

Article Title: Truncation of Fibronectin-Binding Proteins in Staphylococcus aureus Strain Newman Leads to Deficient Adherence and Host Cell Invasion Due to Loss of the Cell Wall Anchor Function

doi: 10.1128/IAI.72.12.7155-7163.2004

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: The fnbA and fnbB genes from S. aureus strains Newman D2C (ATCC 25904) and 8325-4 were amplified by PCR using Deep Vent DNA polymerase.

Techniques: Plasmid Preparation, Isolation, Expressing

Journal:

Article Title: Truncation of Fibronectin-Binding Proteins in Staphylococcus aureus Strain Newman Leads to Deficient Adherence and Host Cell Invasion Due to Loss of the Cell Wall Anchor Function

doi: 10.1128/IAI.72.12.7155-7163.2004

Figure Lengend Snippet: Schematic representation of vector construction. pMG8Vec is a modification of pFNBA4 and does not contain most of the fnbA gene but has retained the complete sequence for the signal peptide. pMG8Vec was used as a vector part for the expression of fnbA and fnbB from S. aureus strains Newman and 8325-4. pMG4ExpA is an expression vector for the fnbA gene from S. aureus strain Newman, in which the fnbA gene was ligated into pMG8Vec by PstI and BglII. pMG4ExpB is an expression vector for the fnbB gene from S. aureus strain Newman, constructed by the same procedure as pMG4ExpA. Only restriction sites relevant to the construction of the vector are shown.

Article Snippet: The fnbA and fnbB genes from S. aureus strains Newman D2C (ATCC 25904) and 8325-4 were amplified by PCR using Deep Vent DNA polymerase.

Techniques: Plasmid Preparation, Modification, Sequencing, Expressing, Construct

Journal:

Article Title: Truncation of Fibronectin-Binding Proteins in Staphylococcus aureus Strain Newman Leads to Deficient Adherence and Host Cell Invasion Due to Loss of the Cell Wall Anchor Function

doi: 10.1128/IAI.72.12.7155-7163.2004

Figure Lengend Snippet: S. aureus strain Newman expresses a regular fusion protein of FnBPA and FnBPB and harbors a stop codon at the ends of the C domains of both fnbA and fnbB. (A) Sequence comparison of fnbA and fnbB of strains Newman and 8325-4 at the beginning of the D domain. The protein translation shows a stop codon in fnbA and fnbB from strain Newman at the end of the C domain (asterisks indicate nucleotide sequence identity of all genes; boldface asterisks indicate stop codons). (B) Sequence comparison of the divergent regions of fnbA and fnbB of strains Newman and 8325-4, respectively. The sequence of fnbANewman was exchanged for that of fnbBNewman from the beginning of the C domain to the end of the M domain. Boxed sequences are 100% identical (asterisks indicate nucleotide sequence identity of all genes). (C) Schematic representation of FnBPA and FnBPB of strains Newman and 8325-4, respectively. S indicates signal peptide; further domain designations (A, B, C, Wr, Wc, and M domains) were used as published previously (26). The sequences shown in the figure were obtained from strain Newman D2C, purchased from the ATCC, by directly sequencing genomic PCR products and fnb genes cloned into the expression plasmids. The regions containing the identified stop codons (in fnbA and fnbB) and crossovers (in fnbA) were verified by sequencing of genomic PCR products in all four strains tested, designated Newman and Newman D2C (including the strain used by Wolz et al. and reference isolates for both strains, freshly purchased from the NCTC), respectively.

Article Snippet: The fnbA and fnbB genes from S. aureus strains Newman D2C (ATCC 25904) and 8325-4 were amplified by PCR using Deep Vent DNA polymerase.

Techniques: Sequencing, Comparison, Clone Assay, Expressing

Journal:

Article Title: Truncation of Fibronectin-Binding Proteins in Staphylococcus aureus Strain Newman Leads to Deficient Adherence and Host Cell Invasion Due to Loss of the Cell Wall Anchor Function

doi: 10.1128/IAI.72.12.7155-7163.2004

Figure Lengend Snippet: Surface expression of cell wall-anchored adhesins in transformants a

Article Snippet: The fnbA and fnbB genes from S. aureus strains Newman D2C (ATCC 25904) and 8325-4 were amplified by PCR using Deep Vent DNA polymerase.

Techniques: Expressing, Clinical Proteomics, Agglutination, Control

Journal:

Article Title: Truncation of Fibronectin-Binding Proteins in Staphylococcus aureus Strain Newman Leads to Deficient Adherence and Host Cell Invasion Due to Loss of the Cell Wall Anchor Function

doi: 10.1128/IAI.72.12.7155-7163.2004

Figure Lengend Snippet: FnBPANewman and FnBPBNewman do not mediate adherence of staphylococci to immobilized fibronectin. Adhesins were expressed in strain TM300 by multicopy plasmids. PMMA coverslips were incubated with [3H]thymidine-labeled S. aureus strains Newman and Cowan I and S. carnosus strains TM300, MG4ExpA, MG4ExpB, MG5ExpA, and BS101. The results are means plus SEM of two independent experiments run in quadruplicate. The data are shown as percentages of adherent bacteria compared to the inoculum.

Article Snippet: The fnbA and fnbB genes from S. aureus strains Newman D2C (ATCC 25904) and 8325-4 were amplified by PCR using Deep Vent DNA polymerase.

Techniques: Incubation, Labeling, Bacteria

Journal:

Article Title: Truncation of Fibronectin-Binding Proteins in Staphylococcus aureus Strain Newman Leads to Deficient Adherence and Host Cell Invasion Due to Loss of the Cell Wall Anchor Function

doi: 10.1128/IAI.72.12.7155-7163.2004

Figure Lengend Snippet: Compilation of selected results for FnBP-dependent functions in strain Newman

Article Snippet: The fnbA and fnbB genes from S. aureus strains Newman D2C (ATCC 25904) and 8325-4 were amplified by PCR using Deep Vent DNA polymerase.

Techniques: Adhesive, Mutagenesis, Functional Assay